Optogenetics: hardware enabling neural control by light
We have developed a set of "optogenetic" reagents, fully genetically encoded reagents that, when targeted to specific cells, enable their physiology to be controlled via light. To confront the 3-dimensional complexity of the living brain, enabling the analysis of the circuits that causally drive or support specific neural computations and behaviors, our lab and our collaborators have developed hardware for delivery of light into the brain, enabling control of complexly shaped neural circuits, as well as the ability to combinatorially activate and silence neural activity in distributed neural circuits, even with single-cell, single-spike precision. We anticipate that these tools will enable the systematic analysis of the brain circuits that mechanistically and causally contribute to specific behaviors and pathologies.
Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of ChronosJournal of Neuroscience | 2017
Ronzitti E, Conti R, Zampini V, Tanese D, Foust AJ, Klapoetke N, Boyden ES, Papagiakoumou E, Emiliani V (2017) Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of Chronos, Journal of Neuroscience 37(44):10679-10689.
Acker LC, Pino EN, Boyden ES, Desimone R (2017) Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates, Journal of Visualized Experiments 128:e56330.
Shaun Berry, Shawn Redmond, Paul Robinson, Todd Thorsen, Mordechai Rothschild, and Edward S. Boyden (2017) Fluidic microoptics with adjustable focusing and beam steering for single cell optogenetics, Optics Express 25(14):16825-16839.
Adaptive Fluidic Microoptics for Single Cell Optogenetics, Bio-Optics: Design and Application (BODA): Biochip and optofluidicsOptical Society of America | 2017
Shaun Berry, Shawn Redmond, Paul Robinson, Todd Thorsen, Mordechai Rothschild, Edward S. Boyden (2017) Adaptive Fluidic Microoptics for Single Cell Optogenetics, Bio-Optics: Design and Application (BODA): Biochip and optofluidics, OSA Biophotonics Congress: Optics in the Life Sciences, San Diego, CA.
Rowlands, C.J., Park, D., Bruns, O.T., Piatkevich, K.D., Fukumura, D., Jain, R.K., Bawendi, M.G., Boyden, E.S. and So, P.T. (2017) Wide-field three-photon excitation in biological samples, Light: Science & Applications 6(5):e16255.
Processes for design, construction and utilisation of arrays of light-emitting diodes and light-emitting diode-coupled optical fibres for multi-site brain light deliveryThe Journal of Engineering | 2015
Bernstein, J.G., Allen, B. D., Guerra, A.A., Boyden, E.S. (2015) Processes for design, construction and utilisation of arrays of light-emitting diodes and light-emitting diode-coupled optical fibres for multi-site brain light delivery, The Journal of Engineering 5:177-184.
Alivisatos, A., Andrews, A., Boyden, E. S., Chun, M., Church, G., Deisseroth, K., Donoghue, J., Fraser, S., Lippincott-Schwartz, J., Looger, L., Masmanidis, S., McEuen, P., Nurmikko, A., Park, H., Peterka, D., Reid, C., Roukes, M., Scherer, A., Schnitzer, M., Sejnowski, T., Shepard, K., Tsao, D., Turrigiano, G., Weiss, P., Xu, C., Yuste, R., Zhuang, X. (2013) Nanotools for Neuroscience and Brain Activity Mapping, ACS Nano, 7(3):1850-66.
Zorzos, A. N., Scholvin, J., Boyden, E. S.*, Fonstad, C. G. (2012) Three-dimensional multiwaveguide probe array for light delivery to distributed brain circuits, Optics Letters 37(23):4841-4843. (* corresponding author)
T. Knopfel and E. Boyden, eds. (2012) Optogenetics: Tools for Controlling and Monitoring Neuronal Activity, Progress in Brain Research, vol. 196, Elsevier.
Doroudchi, M. M., Greenberg, K. P., Zorzos, A. N., Hauswirth, W. W., Fonstad, C. G., Horsager, A., Boyden, E. S. (2011) Towards Optogenetic Sensory Replacement, Conference Proceedings of the IEEE Engineering in Medicine and Biology Society 2011:3139-41.
Boyden, E. S. (2011) Optogenetics. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC ’11).
Boyden, E. S. (2011) The Birth of Optogenetics, The Scientist, July 2011 Issue. (Cover Story.)
Wentz, C. T., Bernstein, J. G., Monahan, P., Guerra, A., Rodriguez, A., Boyden, E. S. (2011) A Wirelessly Powered and Controlled Device for Optical Neural Control of Freely-Behaving Animals, Journal of Neural Engineering 8(4):046021.
Zorzos, A. N., Boyden, E. S.*, and Fonstad, C. G. (2010) Multiwaveguide implantable probe for light delivery to sets of distributed brain targets, Optics Letters 35(24):4133-5. (* corresponding author)
Boyden, E. S., Allen, B. D., Fritz, D. (2010) "Brain Coprocessors." Column, Technology Review. 9/23/2010.
Boyden, E. S. (2010) Controlling the Brain with Light. SPIE Newsroom. 6/10/2010.
Boyden, E. S., Han, X., Talei Franzesi, G., Chan, S., Bernstein, J., Qian, X., Li, M. (2009) "New Techniques for Investigating Brain Rhythms: Optical Neural Control and Multielectrode Recording," In: Rhythms of the Neocortex: Where Do They Come From and What Are They Good For? (Kopell N., ed.) pp. 65-75. Washington, DC: Society for Neuroscience.
Bernstein, J. G., Han, X., Henninger, M. A., Ko, E. Y., Qian, X., Franzesi, G. T., McConnell, J. P., Stern, P., Desimone, R., and Boyden, E. S. (2008) Prosthetic systems for therapeutic optical activation and silencing of genetically-targeted neurons, Proc Soc Photo Opt Instrum Eng 6854:68540H.