Expansion microscopy: physical magnification with nanoscale precision
Publications
Dense, continuous membrane labeling and expansion microscopy visualization of ultrastructure in tissues
Nature Communications | 2025Shin TW, Wang H*, Zhang C*, An B, Lu Y, Zhang E, Lu X, Karagiannis ED, Kang JS, Emenari A, Symvoulidis P, Asano S, Lin L, Costa EK; IMAXT Grand Challenge Consortium; Marblestone AH, Kasthuri N, Tsai LH, Boyden ES (2025) Dense, continuous membrane labeling and expansion microscopy visualization of ultrastructure in tissues, Nature Communications 16(1):1579. (*, contributed equally)
Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain
Nature Communications | 2024Kang J*, Schroeder ME*, Lee Y, Kapoor C, Yu E, Tarr TB, Titterton K, Zeng M, Park D, Niederst E, Wei D, Feng G, Boyden ES (2024) Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain, Nature Communications 15(1):9722. (*, contributed equally)
One-step nanoscale expansion microscopy reveals individual protein shapes
Nature Biotechnology | 2024Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO (2024) One-step nanoscale expansion microscopy reveals individual protein shapes, Nature Biotechnology doi:10.1038/s41587-024-02431-9. Online ahead of print.
Single-shot 20-fold expansion microscopy
Nature Methods | 2024Wang S*, Shin TW*, Yoder HB 2nd, McMillan RB, Su H, Liu Y, Zhang C, Leung KS, Yin P, Kiessling LL**, Boyden ES** (2024) Single-shot 20-fold expansion microscopy, Nature Methods, doi:10.1038/s41592-024-02454-9. Online ahead of print. (*, equal contribution; **, co-corresponding authors)
Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding
Science Translational Medicine | 2024Valdes PA, Yu CJ*, Aronson J*, Ghosh D, Zhao Y, An B, Bernstock JD, Bhere D, Felicella MM, Viapiano MS, Shah K, Chiocca EA**, Boyden ES** (2024) Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding, Science Translational Medicine 16(732):eabo0049. (*, contributed equally; **, contributed equally)
Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs
PLoS One | 2023Cui Y*, Yang G*, Goodwin DR, O'Flanagan CH, Sinha A, Zhang C, Kitko KE, Shin TW, Park D, Aparicio S; CRUK IMAXT Grand Challenge Consortium; Boyden ES (2023) Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs, PLoS One 18(9):e0291506. (*, contributed equally)
Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy
Nature Biomedical Engineering | 2022Deblina Sarkar*, Jinyoung Kang*, Asmamaw T. Wassie*, Margaret E. Schroeder, Zhuyu Peng, Tyler B. Tarr, Ai-Hui Tang, Emily D. Niederst, Jennie Z. Young, Hanquan Su, Demian Park, Peng Yin, Li-Huei Tsai**, Thomas A. Blanpied** & Edward S. Boyden** (2022) Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy, Nature Biomedical Engineering 6:1057–1073. (*, contributed equally; **, co-corresponding)
ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy
Methods in Molecular Biology | 2022Yu CJ*, Orozco Cosio DM*, Boyden ES (2022) ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy, Methods in Molecular Biology 2468:141-203. (* contributed equally)
Nanoscale imaging of clinical specimens using conventional and rapid expansion pathology
Nature Protocols | 2020Bucur O*, Fu F*, Calderon M, Mylvaganam GH, Ly NL, Day J, Watkin S, Walker BD, Boyden ES**, Zhao Y** (2020) Nanoscale imaging of clinical specimens using conventional and rapid expansion pathology, Nature Protocols 15:1649-1672. (*, equal contribution, **, co-corresponding)
Expansion microscopy of C. elegans
eLife | 2020Yu CJ, Barry NC, Wassie AT, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman MB, Haspel G, Boyden ES (2020) Expansion microscopy of C. elegans, eLife 9:e46249.