Expansion microscopy: physical magnification with nanoscale precision

Project

Tools for mapping the molecules and structure of the brain

View Project

Publications

One-step nanoscale expansion microscopy reveals individual protein shapes

Nature Biotechnology | 2024

Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO (2024) One-step nanoscale expansion microscopy reveals individual protein shapes, Nature Biotechnology doi:10.1038/s41587-024-02431-9. Online ahead of print.

Single-shot 20-fold expansion microscopy

Nature Methods | 2024

Wang S*, Shin TW*, Yoder HB 2nd, McMillan RB, Su H, Liu Y, Zhang C, Leung KS, Yin P, Kiessling LL**, Boyden ES** (2024) Single-shot 20-fold expansion microscopy, Nature Methods, doi:10.1038/s41592-024-02454-9. Online ahead of print. (*, equal contribution; **, co-corresponding authors)

Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues

bioRxiv | 2024

Tay Won Shin, Hao Wang*, Chi Zhang*, Bobae An, Yangning Lu, Elizabeth Zhang, Xiaotang Lu, Emmanouil D Karagiannis, Jeong Seuk Kang, Amauche Emenari, Panagiotis Symvoulidis, Shoh Asano, Leanne Lin, Emma K Costa; IMAXT Grand Challenge Consortium; Adam H Marblestone, Narayanan Kasthuri, Li-Huei Tsai, Edward S Boyden (2024) Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues, bioRxiv 2024.03.07.583776; doi: https://doi.org/10.1101/2024.03.07.583776 (*, equal contribution)

Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding

Science Translational Medicine | 2024

Valdes PA, Yu CJ*, Aronson J*, Ghosh D, Zhao Y, An B, Bernstock JD, Bhere D, Felicella MM, Viapiano MS, Shah K, Chiocca EA**, Boyden ES** (2024) Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding, Science Translational Medicine 16(732):eabo0049. (*, contributed equally; **, contributed equally)

Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy

Nature Biomedical Engineering | 2022

Deblina Sarkar*, Jinyoung Kang*, Asmamaw T. Wassie*, Margaret E. Schroeder, Zhuyu Peng, Tyler B. Tarr, Ai-Hui Tang, Emily D. Niederst, Jennie Z. Young, Hanquan Su, Demian Park, Peng Yin, Li-Huei Tsai**, Thomas A. Blanpied** & Edward S. Boyden** (2022) Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy, Nature Biomedical Engineering 6:1057–1073. (*, contributed equally; **, co-corresponding)

Expansion microscopy of C. elegans

eLife | 2020

Yu CJ, Barry NC, Wassie AT, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman MB, Haspel G, Boyden ES (2020) Expansion microscopy of C. elegans, eLife 9:e46249.

Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

Science | 2021

Alon S*, Goodwin DR*, Sinha A*, Wassie AT*, Chen F*, Daugharthy ER**, Bando Y, Kajita A, Xue AG, Marrett K, Prior R, Cui Y, Payne AC, Yao CC, Suk HJ, Wang R, Yu CJ, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer EPR, Kohman RE, Miller JA, Lein ES, Lako A, Cullen N, Rodig S, Helvie K, Abravanel DL, Wagle N, Johnson BE, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church GM***+, Marblestone AH***, Boyden ES***+ (2021) Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems, Science 371(6528):eaax2656. (* equal contribution, ** key contributions to early stages of project, *** equal contribution, +co-corresponding authors)