Expansion microscopy: physical magnification with nanoscale precision
Publications
Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs
PLoS One | 2023Cui Y*, Yang G*, Goodwin DR, O'Flanagan CH, Sinha A, Zhang C, Kitko KE, Shin TW, Park D, Aparicio S; CRUK IMAXT Grand Challenge Consortium; Boyden ES (2023) Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs, PLoS One 18(9):e0291506. (*, contributed equally)
Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy
Nature Biomedical Engineering | 2022Deblina Sarkar*, Jinyoung Kang*, Asmamaw T. Wassie*, Margaret E. Schroeder, Zhuyu Peng, Tyler B. Tarr, Ai-Hui Tang, Emily D. Niederst, Jennie Z. Young, Hanquan Su, Demian Park, Peng Yin, Li-Huei Tsai**, Thomas A. Blanpied** & Edward S. Boyden** (2022) Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy, Nature Biomedical Engineering 6:1057–1073. (*, contributed equally; **, co-corresponding)
Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology Specimens
bioRxiv | 2021Pablo A. Valdes, Chih-Chieh (Jay) Yu, Jenna Aronson, Yongxin Zhao, Joshua D. Bernstock, Deepak Bhere, Bobae An, Mariano S. Viapiano, Khalid Shah, E. Antonio Chiocca, Edward S. Boyden (2021) Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology Specimens, bioRxiv 2021.12.05.471271; doi: https://doi.org/10.1101/2021.12.05.471271.
ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy
Methods in Molecular Biology | 2022Yu CJ*, Orozco Cosio DM*, Boyden ES (2022) ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy, Methods in Molecular Biology 2468:141-203. (* contributed equally)
Nanoscale imaging of clinical specimens using conventional and rapid expansion pathology
Nature Protocols | 2020Bucur O*, Fu F*, Calderon M, Mylvaganam GH, Ly NL, Day J, Watkin S, Walker BD, Boyden ES**, Zhao Y** (2020) Nanoscale imaging of clinical specimens using conventional and rapid expansion pathology, Nature Protocols 15:1649-1672. (*, equal contribution, **, co-corresponding)
Expansion microscopy of C. elegans
eLife | 2020Yu CJ, Barry NC, Wassie AT, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman MB, Haspel G, Boyden ES (2020) Expansion microscopy of C. elegans, eLife 9:e46249.
Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems
Science | 2021Alon S*, Goodwin DR*, Sinha A*, Wassie AT*, Chen F*, Daugharthy ER**, Bando Y, Kajita A, Xue AG, Marrett K, Prior R, Cui Y, Payne AC, Yao CC, Suk HJ, Wang R, Yu CJ, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer EPR, Kohman RE, Miller JA, Lein ES, Lako A, Cullen N, Rodig S, Helvie K, Abravanel DL, Wagle N, Johnson BE, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church GM***+, Marblestone AH***, Boyden ES***+ (2021) Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems, Science 371(6528):eaax2656. (* equal contribution, ** key contributions to early stages of project, *** equal contribution, +co-corresponding authors)
Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells
Current Protocols in Neuroscience | 2020Zhang C*, Kang JS*, Asano SM, Gao R, Boyden ES (2020) Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells, Current Protocols in Neuroscience 92(1):e96. (*, contributed equally)
Expansion Microscopy of Lipid Membranes
bioRxiv | 2019Emmanouil D. Karagiannis*, Jeong Seuk Kang*, Tay Won Shin, Amauche Emenari, Shoh Asano, Leanne Lin, Emma K. Costa, IMAXT Grand Challenge Consortium, Adam H. Marblestone, Narayanan Kasthuri, Edward S. Boyden (2019) Expansion Microscopy of Lipid Membranes, bioRxiv 829903. (*, equal contribution)
A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy
Nature Nanotechnology | 2021Gao R*, Yu CJ*, Gao L, Piatkevich KD, Neve RL, Munro JB, Upadhyayula S, Boyden ES (2021) A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy, Nature Nanotechnology 16:698–707. (*, equal contribution)