Nathan Klapoetke
Nathan received his BS in Electrical Engineering from the University of Wisconsin-Madison, and then completed his PhD in Biological Engineering at MIT in the Synthetic Neurobiology group, where he developed multicolor optogenetic actuators of great versality, speed, and utility in circuit mapping. He then went on to a postdoctoral position at Janelia Farm.
Publications
Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents
Journal of Biological Chemistry | 2019Cho YK, Park D*, Yang A*, Chen F, Chuong AS, Klapoetke NC, Boyden ES (2019) Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents, Journal of Biological Chemistry 294(11):3806-3821. (*, equal contribution)
Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of Chronos
Journal of Neuroscience | 2017Ronzitti E, Conti R, Zampini V, Tanese D, Foust AJ, Klapoetke N, Boyden ES, Papagiakoumou E, Emiliani V (2017) Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of Chronos, Journal of Neuroscience 37(44):10679-10689.
Hearing the light: neural and perceptual encoding of optogenetic stimulation in the central auditory pathway
Scientific Reports | 2015Guo W, Hight AE, Chen JX, Klapoetke NC, Hancock KE, Shinn-Cunningham BG, Boyden ES, Lee DJ, Polley DB (2015) Hearing the light: neural and perceptual encoding of optogenetic stimulation in the central auditory pathway, Scientific Reports 5:10319.
Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance
Neuron | 2015Madisen, L., Garner, A.R., Shimaoka, D., Chuong, A.S., Klapoetke, N.C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., Gu, H., Mills, M., Cheng, A., Tasic, B., Nguyen, T.N., Sunkin, S.M., Benucci, A., Nagy, A., Miyawaki, A., Helmchen, F., Empson, R.M., Knöpfel, T., Boyden, E.S., Reid, R.C., Carandini, M., Zeng, H. (2015) Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance, Neuron 85(5):889-892.
Noninvasive optical inhibition with a red-shifted microbial rhodopsin
Nature Neuroscience | 2014Chuong, A. S., Miri, M. L.*, Busskamp, V.*, Matthews, G.A.C.*, Acker, L.C.*, Soresnsen, A.T., Young, A., Klapoetke, N. C., Henninger, M.A., Kodandaramaiah, S.B., Ogawa, M., Ramanlal, S. B., Bandler, R. C., Allen, B. D., Forest, C.R., Chow, B.Y., Han, X., Lin, Y., Tye, K.M., Roska, B., Cardin, J.A., Boyden, E. S. (2014) Noninvasive optical inhibition with a red-shifted microbial rhodopsin, Nature Neuroscience 17:1123-1129. (*, equal contribution)
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Nature Methods | 2014Hochbaum, D.R.*, Zhao, Y.*, Farhi, S.L., Klapoetke, N.C., Werley, C.A., Kapoor, V., Zou, P., Kralj, J.M., Maclaurin, D., Smedemark-Margulies, N., Saulnier, J., Boulting, G.L., Straub, C., Cho, Y., Melkonian, M., Wong, G.K.-S., Harrison, D. J., Murthy, V.N., Sabatini, B., Boyden, E.S.**, Campbell, R.E.**, Cohen, A.E. (2014) All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins, Nature Methods, 11(8):825-33. (*, equal contribution, **, jointly directed work)
Independent Optical Excitation of Distinct Neural Populations
Nature Methods | 2014Klapoetke, N. C., Murata, Y., Kim S. S., Pulver, S. R., Birdsey-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., Wang, J., Xie, Y., Yan, Z., Zhang, Y., Chow, B.Y., Surek, B., Melkonian, M., Jayaraman, V., Constantine-Paton, M., Wong, G. K.*, Boyden, E. S.* (2014) Independent Optical Excitation of Distinct Neural Populations, Nature Methods 11:338–346. (* co-corresponding authors)
Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes
Methods in Enzymology | 2011Chow, B. Y.*, Chuong, A. S. *, Klapoetke, N. C. *, Boyden, E. S. (2011) Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes, Methods in Enzymology 497:425-43. (* co-first authors)
A high-light sensitivity optical neural silencer: development, and application to optogenetic control of nonhuman primate cortex
Frontiers in Systems Neuroscience | 2011Han, X.*, Chow, B. Y.*, Zhou, H., Klapoetke, N. C., Chuong, A., Rajimehr, R., Yang, A., Baratta, M. V., Winkle, J., Desimone, R., Boyden, E. S. (2011) A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex, Frontiers in Systems Neuroscience 5:18. (* co-first authors)