Jay (Chih-Chieh) Yu
Jay received a BSE degree in Bioengineering at the University of Pennsylvania, where he worked with Professor Chris Fang-Yen on developing high-throughput imaging technologies for the nematode model C. elegans. He was a PhD student in the group, in Biological Engineering at MIT, and then briefly postdoc’ed in the lab to finish up some projects. In the lab, his work focused on developing and applying novel technologies to understand the nervous system of C. elegans.
Publications
Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding
Science Translational Medicine | 2024Valdes PA, Yu CJ, Aronson J, Ghosh D, Zhao Y, An B, Bernstock JD, Bhere D, Felicella MM, Viapiano MS, Shah K, Chiocca EA, Boyden ES (2024) Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding, Science Translational Medicine 16(732):eabo0049.
Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels
Nature Materials | 2023Günay KA, Chang TL, Skillin NP, Rao VV, Macdougall LJ, Cutler AA, Silver JS, Brown TE, Zhang C, Yu CJ, Olwin BB, Boyden ES, Anseth KS (2023) Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels, Nature Materials 22(6):777-785.
ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy
Methods in Molecular Biology | 2022Yu CJ*, Orozco Cosio DM*, Boyden ES (2022) ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy, Methods in Molecular Biology 2468:141-203. (* contributed equally)
Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology Specimens
bioRxiv | 2021Pablo A. Valdes, Chih-Chieh (Jay) Yu, Jenna Aronson, Yongxin Zhao, Joshua D. Bernstock, Deepak Bhere, Bobae An, Mariano S. Viapiano, Khalid Shah, E. Antonio Chiocca, Edward S. Boyden (2021) Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology Specimens, bioRxiv 2021.12.05.471271; doi: https://doi.org/10.1101/2021.12.05.471271.
Tetra-gel enables superior accuracy in combined super-resolution imaging and expansion microscopy
Scientific Reports | 2021Lee H, Yu CC, Boyden ES, Zhuang X, Kosuri P (2021) Tetra-gel enables superior accuracy in combined super-resolution imaging and expansion microscopy, Scientific Reports 11(1):16944.
A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy
Nature Nanotechnology | 2021Gao R*, Yu CJ*, Gao L, Piatkevich KD, Neve RL, Munro JB, Upadhyayula S, Boyden ES (2021) A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy, Nature Nanotechnology 16:698–707. (*, equal contribution)
Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems
Science | 2021Alon S*, Goodwin DR*, Sinha A*, Wassie AT*, Chen F*, Daugharthy ER**, Bando Y, Kajita A, Xue AG, Marrett K, Prior R, Cui Y, Payne AC, Yao CC, Suk HJ, Wang R, Yu CJ, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer EPR, Kohman RE, Miller JA, Lein ES, Lako A, Cullen N, Rodig S, Helvie K, Abravanel DL, Wagle N, Johnson BE, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church GM***+, Marblestone AH***, Boyden ES***+ (2021) Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems, Science 371(6528):eaax2656. (* equal contribution, ** key contributions to early stages of project, *** equal contribution, +co-corresponding authors)
Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics
Cell | 2020Changyang Linghu*, Shannon L. Johnson*, Pablo A. Valdes, Or A. Shemesh, Won Min Park, Demian Park, Kiryl D. Piatkevich, Asmamaw T. Wassie, Yixi Liu, Bobae An, Stephanie A. Barnes, Orhan T. Celiker, Chun-Chen Yao, Chih-Chieh (Jay) Yu, Ru Wang, Katarzyna P. Adamala, Mark F. Bear, Amy E. Keating and Edward S. Boyden (2020) Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics, Cell 183(6):1682-1698. (*, equal contribution)
Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator
Neuron | 2020Shemesh OA*, Linghu C*, Piatkevich KD*, Goodwin D, Celiker OT, Gritton HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han X, Ahrens MB, Boyden ES (2020) Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator, Neuron 107(3):470-486. (*, equal contribution)
Expansion microscopy of C. elegans
eLife | 2020Yu CJ, Barry NC, Wassie AT, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman MB, Haspel G, Boyden ES (2020) Expansion microscopy of C. elegans, eLife 9:e46249.
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Nature Biotechnology | 2016Tillberg, P.W.*, Chen, F.*, Piatkevich, K.D., Zhao, Y., Yu, C.-C., English, B.P., Gao, L., Martorell, A., Suk, H.-J., Yoshida, F., DeGennaro, E.M., Roossien, D.H., Gong, G., Seneviratne, U., Tannenbaum, S.R., Desimone, R., Cai, D., Boyden, E.S. (2016) Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies, Nature Biotechnology 34:987-992. (*, co-first authors)