Demian Park
Demian Park completed a PhD at Tufts University in Neuroscience, followed by postdoctoral training at Brandeis and MIT. He then worked as a scientist at Phylonix. He has studied the electrophysiology of Drosophila neurons, developed new methods for zebrafish screening, and studied voltage-gated calcium channels. As a research scientist in the Synthetic Neurobiology group, Demian works on novel optogenetic strategies for interfacing to the brain.
Publications
Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs
PLoS One | 2023Cui Y*, Yang G*, Goodwin DR, O'Flanagan CH, Sinha A, Zhang C, Kitko KE, Shin TW, Park D, Aparicio S; CRUK IMAXT Grand Challenge Consortium; Boyden ES (2023) Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs, PLoS One 18(9):e0291506. (*, contributed equally)
Recording of cellular physiological histories along optically readable self-assembling protein chains
Nature Biotechnology | 2023Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang R, Zhang C, Park D, Park WM, Ramirez S, Boyden ES (2023) Recording of cellular physiological histories along optically readable self-assembling protein chains, Nature Biotechnology 41(5):640-651.
Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy
Nature Biomedical Engineering | 2022Deblina Sarkar*, Jinyoung Kang*, Asmamaw T. Wassie*, Margaret E. Schroeder, Zhuyu Peng, Tyler B. Tarr, Ai-Hui Tang, Emily D. Niederst, Jennie Z. Young, Hanquan Su, Demian Park, Peng Yin, Li-Huei Tsai**, Thomas A. Blanpied** & Edward S. Boyden** (2022) Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy, Nature Biomedical Engineering 6:1057–1073. (*, contributed equally; **, co-corresponding)
Rapid directed molecular evolution of fluorescent proteins in mammalian cells
Protein Science | 2021Babakhanova S*, Jung EE*, Namikawa K*, Zhang H*, Wang Y, Subach OM, Korzhenevskiy DA, Rakitina TV, Xiao X, Wang W, Shi J, Drobizhev M, Park D, Eisenhard L, Tang H, Köster RW, Subach FV**, Boyden ES**, Piatkevich KD** (2022) Rapid directed molecular evolution of fluorescent proteins in mammalian cells, Protein Science 31(3):728-751. (*, contributed equally; **, co-corresponding)
Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics
Cell | 2020Changyang Linghu*, Shannon L. Johnson*, Pablo A. Valdes, Or A. Shemesh, Won Min Park, Demian Park, Kiryl D. Piatkevich, Asmamaw T. Wassie, Yixi Liu, Bobae An, Stephanie A. Barnes, Orhan T. Celiker, Chun-Chen Yao, Chih-Chieh (Jay) Yu, Ru Wang, Katarzyna P. Adamala, Mark F. Bear, Amy E. Keating and Edward S. Boyden (2020) Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics, Cell 183(6):1682-1698. (*, equal contribution)
Sparse decomposition light-field microscopy for high speed imaging of neuronal activity
Optica | 2020Young-Gyu Yoon*, Zeguan Wang*, Nikita Pak, Demian Park, Peilun Dai, Jeong Seuk Kang, Ho-Jun Suk, Panagiotis Symvoulidis, Burcu Guner-Ataman, Kai Wang**, and Edward S. Boyden** (2020) Sparse decomposition light-field microscopy for high speed imaging of neuronal activity, Optica 7(10):1457-1468. (* equal contributors, ** equal contributors)
An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques
Neuron | 2020Gong X, Mendoza-Halliday D, Ting JT, Kaiser T, Sun X, Bastos AM, Wimmer RD, Guo B, Chen Q, Zhou Y, Pruner M, Wu CW, Park D, Deisseroth K, Barak B, Boyden ES, Miller EK, Halassa MM, Fu Z, Bi G, Desimone R, Feng G (2020) An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques, Neuron 107(1):38-51.
Population imaging of neural activity in awake behaving mice
Nature | 2019Piatkevich KD*, Bensussen S*, Tseng HA*, Shroff SN, Lopez-Huerta VG, Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa E, Sabatini BL, Fu Z, Boyden ES**, Han X** (2019) Population imaging of neural activity in awake behaving mice, Nature 574:413–417. (* equal contribution, **, co-corresponding)
Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes
Nature Communications | 2019Guo SM, Veneziano R, Gordonov S, Li L, Danielson E, Perez de Arce K, Park D, Kulesa AB, Wamhoff EC, Blainey PC, Boyden ES, Cottrell JR, Bathe M (2019) Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes, Nature Communications 10(1):4377.
Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents
Journal of Biological Chemistry | 2019Cho YK, Park D*, Yang A*, Chen F, Chuong AS, Klapoetke NC, Boyden ES (2019) Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents, Journal of Biological Chemistry 294(11):3806-3821. (*, equal contribution)
A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Nature Chemical Biology | 2018Piatkevich KD*, Jung EE*, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES (2018) A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters, Nature Chemical Biology 14(4):352-360. (*, co-first authors)
Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues
Nano Letters | 2017Wang Y, Woehrstein JB, Donoghue N, Dai M, Avendaño MS, Schackmann RCJ, Zoeller JJ, Wang SSH, Tillberg PW, Park D, Lapan SW, Boyden ES, Brugge JS, Kaeser PS, Church GM, Agasti SS, Jungmann R, Yin P (2017) Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues, Nano Letters 17(10):6131-6139.
Wide-field three-photon excitation in biological samples
Light: Science & Applications | 2017Rowlands, C.J., Park, D., Bruns, O.T., Piatkevich, K.D., Fukumura, D., Jain, R.K., Bawendi, M.G., Boyden, E.S. and So, P.T. (2017) Wide-field three-photon excitation in biological samples, Light: Science & Applications 6(5):e16255.