Asmamaw (Oz) Wassie
Asmamaw “Oz” Wassie completed a BS in Biological Engineering at MIT. As a PhD student in Biological Engineering, and funded by a Hertz Foundation Fellowship, he pursued new physical, chemical, and biological methods for controlling and observing neural processes. After completing his PhD, and briefly postdoc’ing, he became scientific director of a startup company in the biotech space.
Publications
Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy
Nature Biomedical Engineering | 2022Deblina Sarkar*, Jinyoung Kang*, Asmamaw T. Wassie*, Margaret E. Schroeder, Zhuyu Peng, Tyler B. Tarr, Ai-Hui Tang, Emily D. Niederst, Jennie Z. Young, Hanquan Su, Demian Park, Peng Yin, Li-Huei Tsai**, Thomas A. Blanpied** & Edward S. Boyden** (2022) Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy, Nature Biomedical Engineering 6:1057–1073. (*, contributed equally; **, co-corresponding)
Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses
Nucleic Acids Research | 2021Liu S, Punthambaker S, Iyer EPR, Ferrante T, Goodwin D, Fürth D, Pawlowski AC, Jindal K, Tam JM, Mifflin L, Alon S, Sinha A, Wassie AT, Chen F, Cheng A, Willocq V, Meyer K, Ling KH, Camplisson CK, Kohman RE, Aach J, Lee JH, Yankner BA, Boyden ES, Church GM (2021) Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses, Nucleic Acids Research 49(10):e58.
Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems
Science | 2021Alon S*, Goodwin DR*, Sinha A*, Wassie AT*, Chen F*, Daugharthy ER**, Bando Y, Kajita A, Xue AG, Marrett K, Prior R, Cui Y, Payne AC, Yao CC, Suk HJ, Wang R, Yu CJ, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer EPR, Kohman RE, Miller JA, Lein ES, Lako A, Cullen N, Rodig S, Helvie K, Abravanel DL, Wagle N, Johnson BE, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church GM***+, Marblestone AH***, Boyden ES***+ (2021) Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems, Science 371(6528):eaax2656. (* equal contribution, ** key contributions to early stages of project, *** equal contribution, +co-corresponding authors)
Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics
Cell | 2020Changyang Linghu*, Shannon L. Johnson*, Pablo A. Valdes, Or A. Shemesh, Won Min Park, Demian Park, Kiryl D. Piatkevich, Asmamaw T. Wassie, Yixi Liu, Bobae An, Stephanie A. Barnes, Orhan T. Celiker, Chun-Chen Yao, Chih-Chieh (Jay) Yu, Ru Wang, Katarzyna P. Adamala, Mark F. Bear, Amy E. Keating and Edward S. Boyden (2020) Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics, Cell 183(6):1682-1698. (*, equal contribution)
Expansion microscopy of C. elegans
eLife | 2020Yu CJ, Barry NC, Wassie AT, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman MB, Haspel G, Boyden ES (2020) Expansion microscopy of C. elegans, eLife 9:e46249.
Expansion microscopy: principles and uses in biological research
Nature Methods | 2018Wassie AT*, Zhao Y*, Boyden ES (2019) Expansion microscopy: principles and uses in biological research, Nature Methods 16(1):33-41. (*, equal contributors)
Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues
Current Protocols in Cell Biology | 2018Asano SM*, Gao R*, Wassie AT*, Tillberg PW, Chen F, Boyden ES (2018) Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues, Current Protocols in Cell Biology 80(1):e56. (*, co-first authors)
Iterative expansion microscopy
Nature Methods | 2017Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.
Nanoscale imaging of RNA with expansion microscopy
Nature Methods | 2016Chen, F.*, Wassie, A.T.*, Cote, A.J., Sinha, A., Alon, S., Asano, S., Daugharthy, E.R., Chang, J.-B., Marblestone, A., Church, G.M., Raj, A., Boyden, E.S. (2016) Nanoscale Imaging of RNA with Expansion Microscopy, Nature Methods 13(8):679-84. (*, co-first authors)