Fully-automated, in-vivo, single cell electrophysiology

J. Go, A. Fan, C. Lu, S.B. Kodandaramaiah, G.L. Holst, W. Stoy, I. Kolb, E.S. Boyden, C.R. Forest (2013) Fully-automated, in-vivo, single cell electrophysiology, Proceedings of the 28th Annual Meeting of the American Society for Precision Engineering, Saint Paul, MN, Oct 20-25, 2013.

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In this work, we report progress in developing
a device that allows fully autonomous sequential patch
clamp experimentation. The
machine works by integrating a storage
magazine of pre-filled pipettes that can be
accessed, and swapped, by the headstage at
the conclusion of each experiment. In operation,
following each neuron measurement, the
program enters “swap” state where a set of
programmed actuator movements take place.
First, the headstage translates towards the
pipette storage assembly and deposits its used
pipette. The storage assembly rotates to index a
fresh pipette, its is grasped, and finally, the
headstage returns to its previously designated
home position in preparation of subsequent



Tools for recording high-speed brain dynamics

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