Shahar Alon

Shahar received a BSc degree in Physics from Bar-Ilan University, and an MSc degree in Neurobiology and a PhD degree in Neuroscience, both from Tel Aviv University. In his PhD he studied the functional roles of RNA editing in the brain. During his doctoral training he was supported by a Clore fellowship and his postdoctoral training was supported by a Rothschild fellowship. During his time as a postdoctoral scholar at MIT, Shahar combined tools from computational biology, physics, and molecular biology to design new ways to map and analyze brain function. He received a Life Sciences Research Foundation fellowship to support his work in the Boyden lab. After completing his postdoc, he went on to a tenure track faculty job at Bar Ilan University.

Publications

Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses

Nucleic Acids Research | 2021

Liu S, Punthambaker S, Iyer EPR, Ferrante T, Goodwin D, Fürth D, Pawlowski AC, Jindal K, Tam JM, Mifflin L, Alon S, Sinha A, Wassie AT, Chen F, Cheng A, Willocq V, Meyer K, Ling KH, Camplisson CK, Kohman RE, Aach J, Lee JH, Yankner BA, Boyden ES, Church GM (2021) Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses, Nucleic Acids Research 49(10):e58.

Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

Science | 2021

Alon S*, Goodwin DR*, Sinha A*, Wassie AT*, Chen F*, Daugharthy ER**, Bando Y, Kajita A, Xue AG, Marrett K, Prior R, Cui Y, Payne AC, Yao CC, Suk HJ, Wang R, Yu CJ, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer EPR, Kohman RE, Miller JA, Lein ES, Lako A, Cullen N, Rodig S, Helvie K, Abravanel DL, Wagle N, Johnson BE, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church GM***+, Marblestone AH***, Boyden ES***+ (2021) Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems, Science 371(6528):eaax2656. (* equal contribution, ** key contributions to early stages of project, *** equal contribution, +co-corresponding authors)

Nanoscale imaging of RNA with expansion microscopy

Nature Methods | 2016

Chen, F.*, Wassie, A.T.*, Cote, A.J., Sinha, A., Alon, S., Asano, S., Daugharthy, E.R., Chang, J.-B., Marblestone, A., Church, G.M., Raj, A., Boyden, E.S. (2016) Nanoscale Imaging of RNA with Expansion Microscopy, Nature Methods 13(8):679-84. (*, co-first authors)