Paul Reginato

Paul, while in the group, worked on technology that integrates the high-dimensional ‘omic’ data that characterizes modern biology, with the 3-dimensional reality of biological systems. The big picture goal, in collaboration with many others, of his thesis work was to know the function and location of biomolecules of interest throughout tissues — that is, obtaining comprehensive molecular maps of tissues. To approach this goal, he applied and extended fluorescent in situ sequencing (FISSEQ) and expansion microscopy (ExM) technologies, towards the 3-d analysis of the structure of the genome. Paul completed a BS degree in Biology and Mathematics from the University of Waterloo. As a Biological Engineering PhD student at the Massachusetts Institute of Technology, Paul worked in the Boyden lab and the Church lab (Harvard) on novel biotechnologies.

Publications

Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

Science | 2021

Alon S*, Goodwin DR*, Sinha A*, Wassie AT*, Chen F*, Daugharthy ER**, Bando Y, Kajita A, Xue AG, Marrett K, Prior R, Cui Y, Payne AC, Yao CC, Suk HJ, Wang R, Yu CJ, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer EPR, Kohman RE, Miller JA, Lein ES, Lako A, Cullen N, Rodig S, Helvie K, Abravanel DL, Wagle N, Johnson BE, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church GM***+, Marblestone AH***, Boyden ES***+ (2021) Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems, Science 371(6528):eaax2656. (* equal contribution, ** key contributions to early stages of project, *** equal contribution, +co-corresponding authors)

In situ genome sequencing resolves DNA sequence and structure in intact biological samples

Science | 2020

Payne AC*, Chiang ZD*, Reginato PL*, Mangiameli SM, Murray EM, Yao CC, Markoulaki S, Earl AS, Labade AS, Jaenisch R, Church GM, Boyden ES**, Buenrostro JD**, Chen F** (2020) In situ genome sequencing resolves DNA sequence and structure in intact biological samples, Science 371(6532):eaay3446. (* equal contribution, **co-corresponding)

Expansion Microscopy of Zebrafish for Neuroscience and Developmental Biology Studies

Proceedings of the National Academy of Sciences | 2017

Freifeld L, Odstrcil I, Förster D, Ramirez A, Gagnon JA, Randlett O, Costa EK, Asano S, Celiker OT, Gao R, Martin-Alarcon DA, Reginato P, Dick C, Chen L, Schoppik D, Engert F, Baier H, Boyden ES (2017) Expansion microscopy of zebrafish for neuroscience and developmental biology studies, Proceedings of the National Academy of Sciences 114(50):E10799-E10808.